BRCA2 Knockout cell line(PC-3M)

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location： Home > Products > KO Cell Lines > BRCA2 Knockout cell line(PC-3M)

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BRCA2 Knockout cell line(PC-3M) Order

BRCA2 Knockout cell line(PC-3M)
Catalog#
YKO-HJ098
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BRCA2 Knockout cell line(PC-3M)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BRCA2 Knockout cell line (PC-3M)
Species
Human
Gene Name
BRCA2
Gene ID
675
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 7 (based on transcript BRCA2-201)
Validation: PCR+Sanger Sequencing
BRCA2 Knockout cell line(PC-3M) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BRCA2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC-3M cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BRCA2 gene knockout BRCA2 Knockout cell line (PC-3M) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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